#154234

2C4-Gamma2A cell line

Please Enter Your Email ID
Email ID

Cat. #154234

2C4-Gamma2A cell line

Cat. #: 154234

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Disease: Cancer

Model: Mutant

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: George Stark ; Ian Kerr

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 2C4-Gamma2A cell line
  • Cancer: Sarcoma
  • Cancers detailed: Fibrosarcoma
  • Research fields: Cell signaling and signal transduction;Genetics;Immunology
  • Tool sub type: Continuous
  • Parental cell: 2C4 cell line (a derivative of HT 1080 cell line)
  • Organism: Human
  • Disease: Cancer
  • Model: Mutant
  • Description: Mutant gamma2A cells retain a wild-type interferon (IFN)-alpha/beta response (e.g., class I HLA Binding of IFN-gamma to gamma2A cells is normal), but the cells lack detectable JAK2 mRNA and protein and are completely defective in all aspects of the IFN-gamma response. More specifically, they are defective in IFN-gamma inducible activation of the JAK/STAT pathway, gene expression, and an antiviral state with respect to encephalomyocarditis (EMC) virus. In all cases, JAK2 (but not JAK1 or Tyk2 ...
  • Production details: The 2C4-gamma2A cell line is a derivative of the 2C4 cell line which is a clonal derivative of the human fibrosarcoma cell line HT 1080. The 2C4 cell line clone expresses the simple cell-surface marker CD2 (normally expressed only on T cells) under the control of the 9-27 gene promoter. The 9-27 gene promoter is inducible by interferon gamma; fluorescence-activated cell sorter (FACS) analysis was used to screen cells for loss or gain of interferon gamma inducibility of CD2 expression. Cell li...
  • Biosafety level: 1
  • Recommended controls: 2C4 parental line

Target Details

  • Target: Interferon gamma; JAK2

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10% heat-inactivated FBS + 400ug G418 per ml
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Gamma2A Jak2 Cell Line ; 2C4 cell line

References

  • Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
  • A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
  • Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
  • Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.