#154462

16E6/E7-HSK cell line

Cat. #154462

16E6/E7-HSK cell line

Cat. #: 154462

Sub-type: Primary

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Skin

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Aloysius Klingelhutz

Institute: The University of Iowa

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 16E6/E7-HSK cell line
  • Research fields: Cancer
  • Tool sub type: Primary
  • Parental cell: Keratinocytes isolated from punch biopsies of the skin from the buttocks
  • Organism: Human
  • Tissue: Skin
  • Disease: Cancer
  • Growth properties: Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture
  • Conditional: No
  • Description: Expresses skin cell markers; proinflammatory. Tissue is adult skin cells.
  • Production details: Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418
  • Biosafety level: 1

Target Details

  • Target: Used as an immortal adult human skin keratinocyte line for pathogenesis and inflammation studies

Handling

  • Format: Frozen
  • Growth medium: G418 selection.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
  • Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA compon