Cat. #151759
C80 Colorectal adenocarcinoma Cell Line
Cat. #: 151759
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: C80 Colorectal adenocarcinoma Cell Line
- Alternate name: C80; c80; C 80; C-80
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Dukes' stage D;Human colorectal adenocarcinoma;Moderately well differentiated
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C80 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage D.
- Cellosaurus id: CVCL_5249
Handling
- Format: Frozen
- Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Cells grow as small dense islands and will not form a monolayer. C80 cells grow very slowly. Recommended that the cell line should be subcultured approximately every 5 days. On resuscitation, centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 2-4x10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO ; 37°C.
References
- Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.
