Cat. #162412
Monoclonal antibody anti-Dimethyl K-PhosphoS Histone H3 (H3-2H12)
Cat. #: 162412
Target: Dimethyl K-PhosphoS Histone H3
Class: Monoclonal
Application: ELISA, WB, IF, immunoprecipitation
Reactivity: Sequence conserved in wide range of species
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Primary Citation: Eberlin et al. (2008). Mol Cell Biol. 28(5):1739-54. PMID: 18180282
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Monoclonal antibody anti-Dimethyl K-PhosphoS Histone H3 (H3-2H12)
- Research fields: Epigenetics and Nuclear signalling
- Clone: 51TA-2H12
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Sequence conserved in wide range of species
- Host: Mouse
- Application: ELISA, WB, IF, immunoprecipitation
- Description: Antibody to Dimethyl K-PhosphoS Histone H3. The generated 51TA2H12 clone recognizes S10- or S28-phosphorylated histone H3, but only when doubly modified with H3K9me2 or H3K27me2 (see reference).Specificity: dimethylated (K9)-phosphorylated (S10) histone H3 or dimethylated (K27)- phosphorylated (S28) histone H3. These histone H3 modifications are correlated with mitosis.
- Immunogen: Ovalbumin conjugated synthetic peptide ASGSSRSKS[dimethyl K]DRKYTL(C) corresponding to aa 180-195 of human TAF10.
- Isotype: IgG1, K
- Additional notes: Avoid repeat freezing and thawing cycles. Does not contain any preservative.
Target Details
- Target: Dimethyl K-PhosphoS Histone H3
- Target background: Histone H3 is both dimethylated on a lysine and phosphorylated on a serine, a combination of modifications that can have specific effects on chromatin and gene regulation in cancer.
Applications
- Application: ELISA, WB, IF, immunoprecipitation
- Application notes: Available as crude ascites fluid; Recommended dilutions: 1/500-1/5000
Handling
- Storage conditions: -20°C (up to 3 years)
References
- Eberlin et al. (2008). Mol Cell Biol. 28(5):1739-54, PMID: 18180282
