Creatine Kinase B (CK-BYK) [2E10]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Creatine Kinase B (CK-BYK) [2E10]
• Alternate name: CKBB, creatine phosphokinase, phosphocreatine kinase
• Research fields: Cell biology;Metabolism;Neurobiology
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human ; Mouse ; Rat ; Rabbit
• Host: Mouse
• Application: WB ; EM ; IHC ; IF ; WB
• Description: Creatine kinase, also known as creatine phosphokinase or phosphocreatine kinase, is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and uses adenosine triphosphate to create phosphocreatine and adenosine diphosphate. Subcellular partitioning of creatine kinase contributes to the formation of patterns in intracellular ATP distribution and the fuelling of cellular processes with a high and sudden energy demand. We have previously shown that brain-type creatine kinase (CK-B) accumulates at the phagocytic cup in macrophages where it is involved in the compartmentalized generation of ATP for actin remodeling.
• Immunogen: A synthetic 17-mer peptide corresponding to a unique sequence in the amino-terminal region of human creatine kinase B
• Isotype: IgG2b
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: Brain-type creatine kinase
• Target background: Creatine kinase, also known as creatine phosphokinase or phosphocreatine kinase, is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and uses adenosine triphosphate to create phosphocreatine and adenosine diphosphate. Subcellular partitioning of creatine kinase contributes to the formation of patterns in intracellular ATP distribution and the fuelling of cellular processes with a high and sudden energy demand. We have previously shown that brain-type creatine kinase (CK-B) accumulates at the phagocytic cup in macrophages where it is involved in the compartmentalized generation of ATP for actin remodeling.

Applications

• Application: WB ; EM ; IHC ; IF ; WB

Handling

• Format: Liquid
• Unit size: 100 ug
• Shipping conditions: Dry ice

References

• Venter et al. 2015. Eur J Cell Biol. 94(2):114-27. PMID: 25538032.
• Sistermans et al. 1995. Cell Tissue Res. 280(2):435-46. PMID: 7781040.
• de Kok et al. 1995. Mol Cell Biochem. 143(1):59-65. PMID: 7776959.