Cat. #151512
Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
Cat. #: 151512
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Zebrafish gut absorptive cell epitopes
Class: Monoclonal
Application: IHC ; IP ; WB
Reactivity: Zebrafish
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Linda Ariza-McNaughton
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Colorectal
- Research fields: Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology;Tags and cell markers
- Clone: FIS 4E8/1; 2F11
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 200 kDa
- Strain: Balb/c
- Reactivity: Zebrafish
- Host: Mouse
- Application: IHC ; IP ; WB
- Description: This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
- Immunogen: Lysate of zebrafish intestines
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Zebrafish gut absorptive cell epitopes
- Molecular weight: 200 kDa
- Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli).
Applications
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1] ; Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]
References
- Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
- Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
- Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.
- Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.
- Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.
- Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
- Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
- Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.
