Anti-V(beta)3 T cell receptor (TcR) [JOVI.3]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-V(beta)3 T cell receptor (TcR) [JOVI.3]
• Research fields: Cell biology;Immunology;Immuno-oncology;Immunophenotyping
• Clone: JOVI.3
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IP ; Fn
• Description: Monoclonal antibody used in the study of T cell diseases, and as a CAR therapy in T cell lymphoma/leukaemia. Background and Research Application The T cell receptor is heterodimer of alpha/beta or gamma/delta chains, expressed on all mature T cells. TCR's recognise antigen peptides bound to MHC molecules, providing the basis of antigen specific response by T cells. JOVI.3 can be used for studies of T cell mediated diseases, including autoimmunity and allergy. The antibody recognises any human T cell receptor that has the V beta 3 region, staining 50-75% of peripheral blood T cells and T cell lines.
• Immunogen: Thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR.
• Immunogen uniprot id: P09693
• Isotype: IgG2a
• Myeloma used: P3/NS1/1-Ag4.1
• Recommended controls: CD3 positive PBMCs

Target Details

• Target: V(beta)3 T cell receptor (TcR)
• Tissue cell line specificity: CD3 positive PBMCs
• Target background: Monoclonal antibody used in the study of T cell diseases, and as a CAR therapy in T cell lymphoma/leukaemia. Background and Research Application The T cell receptor is heterodimer of alpha/beta or gamma/delta chains, expressed on all mature T cells. TCR's recognise antigen peptides bound to MHC molecules, providing the basis of antigen specific response by T cells. JOVI.3 can be used for studies of T cell mediated diseases, including autoimmunity and allergy. The antibody recognises any human T cell receptor that has the v?3 region, staining 50-75% % of peripheral blood T cells and T cell lines.

Applications

• Application: FACS ; IHC ; IP ; Fn

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

• Viguier et al. 2015. J Invest Dermatol. 135(2):418-24. PMID: 25207820.
• Peripheral and local human papillomavirus 16-specific CD8+ T-cell expansions characterize erosive oral lichen planus.
• San Jos et al. 1999. J Biol Chem. 274(47):33740-6. PMID: 10559266.
• Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway.
• Sahuquillo et al. 1998. J Exp Med. 187(8):1179-92. PMID: 9547330.
• T cell receptor (TCR) engagement in apoptosis-defective, but interleukin 2 (IL-2)-producing, T cells results in impaired ZAP70/CD3-zeta association.
• Viney et al. 1992. Hybridoma. 11(6):701-13. PMID: 1284120.
• Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.