#151870

Anti-TIGAR [M2-P4H2]

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Cat. #151870

Anti-TIGAR [M2-P4H2]

Cat. #: 151870

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-5 days

Target: TP53-induced glycolysis and apoptosis regulator (TIGAR)

Class: Monoclonal

Application: ELISA ; IHC ; IF ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ayham Alnabulsi

Institute: Vertebrate Antibodies Limited

Tool Details
Target Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-TIGAR [M2-P4H2]
  • Research fields: Apoptosis and autophagy;Cancer;Genetics
  • Clone: M2-P4H2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; WB
  • Description: TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose- 2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose- 2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis.
  • Immunogen: Synthetic peptide; IINFEEGREV (amino acids 239-248)
  • Isotype: IgG1 kappa
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma

Target Details

  • Target: TP53-induced glycolysis and apoptosis regulator (TIGAR)
  • Tissue cell line specificity: ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma
  • Target background: TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose-2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose-2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis.

Applications

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C