Cat. #153646
Anti-Protein Kinase C a [133]
Cat. #: 153646
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Protein Kinase C a
Class: Monoclonal
Application: IHC ; WB
Reactivity: Human ; Mammals
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: BioServ UK Ltd
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Protein Kinase C a [133]
- Alternate name: Protein kinase C alpha, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
- Research fields: Cancer;Neurobiology
- Clone: 133
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 75 kDa
- Strain: Balb/c
- Reactivity: Human ; Mammals
- Host: Mouse
- Application: IHC ; WB
- Description: Monoclonal antibody which specifically detects the ? isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKC?) is involved in the regulation of cell proliferation during cell cycle progression. PKC-? is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the ? isoform of PKC and binds to a sequence at the C terminus of PKC? to detect its expression in vitro.
- Immunogen: Recognizes sequence PQFVHPILQSAV at the C terminus at PKCa
- Immunogen uniprot id: P17252
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Retina
Target Details
- Target: Protein Kinase C a
- Molecular weight: 75 kDa
- Tissue cell line specificity: Retina
- Target background: Monoclonal antibody which specifically detects the a isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKCa) is involved in the regulation of cell proliferation during cell cycle progression. PKC-a is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the a isoform of PKC and binds to a sequence at the C terminus of PKCa to detect its expression in vitro.
Applications
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.
- Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.
- Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.
- Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.
- Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.
- Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.
- Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Ca consensus site.
- Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.
- Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
- Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.
- High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.
- Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.
- Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.
