Anti-Protein Kinase C a [133]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Protein Kinase C a [133]
• Alternate name: Protein kinase C alpha, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
• Research fields: Cancer;Neurobiology
• Clone: 133
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 75 kDa
• Strain: Balb/c
• Reactivity: Human ; Mammals
• Host: Mouse
• Application: IHC ; WB
• Description: Monoclonal antibody which specifically detects the ? isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKC?) is involved in the regulation of cell proliferation during cell cycle progression. PKC-? is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the ? isoform of PKC and binds to a sequence at the C terminus of PKC? to detect its expression in vitro.
• Immunogen: Recognizes sequence PQFVHPILQSAV at the C terminus at PKCa
• Immunogen uniprot id: P17252
• Isotype: IgG2a
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Retina

Target Details

• Target: Protein Kinase C a
• Molecular weight: 75 kDa
• Tissue cell line specificity: Retina
• Target background: Monoclonal antibody which specifically detects the a isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKCa) is involved in the regulation of cell proliferation during cell cycle progression. PKC-a is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the a isoform of PKC and binds to a sequence at the C terminus of PKCa to detect its expression in vitro.

Applications

• Application: IHC ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

• De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.
• Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.
• Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.
• Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.
• Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.
• Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.
• Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Ca consensus site.
• Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.
• Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
• Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.
• High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.
• Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.
• Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.