Anti-Phospho-Raf1 (Ser338) [DPR/2/2A/3A]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Phospho-Raf1 (Ser338) [DPR/2/2A/3A]
• Alternate name: RAF1; Raf-1; c-Raf; NS5; CRAF; v-raf-1 murine leukemia viral oncogene homolog 1
• Research fields: Cancer;Immunology
• Clone: DPR/2/2A/3A
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Cell signalling pathway: MAPK/ERK
• Reactivity: Human
• Host: Rat
• Application: WB
• Description: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
• Immunogen: Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde
• Isotype: IgG1

Target Details

• Target: Raf-1
• Target background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Applications

• Application: WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Chiloeches et al. 2001. Mol Cell Biol. 21(7):2423-34. PMID: 11259591.
• S338 phosphorylation of Raf-1 is independent of phosphatidylinositol 3-kinase and Pak3.
• Mason et al. 1999. EMBO J. 18(8):2137-48. PMID: 10205168.
• Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.