Anti-MMP3 [1B4]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-MMP3 [1B4]
• Research fields: Cancer;Cell biology;Genetics;Tissue-specific biology
• Clone: 1B4
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: ELISA ; IHC ; IF ; IP ; WB
• Description: Human matrix metalloproteinase 3 (MMP-3) is a member of the matrix metalloproteinases (MMP) family that are peptidases involved in the breakdown of extracellular matrix proteins. MMP-3 degrades fibronectin, laminin, gelatins of type I, III, IV, and V; collagen of type II, III, IV, X, and IX, and proteoglycans. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is also thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
• Immunogen: Ovalbumin-conjugated synthetic peptide; CKSLRKLEPELH
• Isotype: IgG1
• Myeloma used: P3X63Ag8.653
• Recommended controls: IHC: formalin-fixed, paraffin-embedded lung containing intra-alveolar macrophageswestern blot: rhMMP-3, 400 ng per lane

Target Details

• Target: Human matrix metalloproteinase 3 (MMP-3)
• Tissue cell line specificity: IHC: formalin-fixed, paraffin-embedded lung containing intra-alveolar macrophageswestern blot: rhMMP-3, 400 ng per lane
• Target background: Human matrix metalloproteinase 3 (MMP-3) is a member of the matrix metalloproteinases (MMP) family that are peptidases involved in the breakdown of extracellular matrix proteins. MMP-3 degrades fibronectin, laminin, gelatins of type I, III, IV, and V; collagen of type II, III, IV, X, and IX, and proteoglycans. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is also thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.

Applications

• Application: ELISA ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -80° C
• Shipping conditions: Dry ice

References

• Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.
• The matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase profile in colorectal polyp cancers.
• Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.
• Profiling markers of prognosis in colorectal cancer.
• Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.
• Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers.
• Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.
• Matrix metalloproteinases and their inhibitors in gastric cancer.
• Murray et al. 1998. J Pathol. 185(3):256-61. PMID: 9771478.
• Matrix metalloproteinase-1 is associated with poor prognosis in oesophageal cancer.