Cat. #152939
Anti-Lewis y/b hapten [NCRC37]
Cat. #: 152939
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Sialyltetraosylceramiden antigen on colorectal tumor cell surface
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; Fn
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lindy Durrant
Institute: University of Nottingham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Lewis y/b hapten [NCRC37]
- Research fields: Immunology;Tissue-specific biology
- Clone: NCRC37
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; Fn
- Description: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
- Immunogen: Tissue cultured colorectal tumor cell lines (C170, C146, Colo205, and JW)
- Isotype: IgG1
- Myeloma used: NS0
- Recommended controls: C14 (NCRC10)
Target Details
- Target: Sialyltetraosylceramiden antigen on colorectal tumor cell surface
- Tissue cell line specificity: C14 (NCRC10)
- Target background: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Applications
- Application: ELISA ; FACS ; IHC ; Fn
Handling
- Format: Liquid
- Concentration: 1.14 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Durrant et al. 2006. Cancer Res. 66(11):5901-9. PMID: 16740730.
- A new anticancer glycolipid monoclonal antibody, SC104, which directly induces tumor cell apoptosis.
- Durrant et al. 1993. Hybridoma. 12(6):647-60. PMID: 7507082.
- Development of second generation monoclonal antibodies recognising Lewisy/b antigen by anti-idiotypic immunisation.
