Cat. #151121
Anti-Keratin14 [LL001]
Cat. #: 151121
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Keratin 14
Class: Monoclonal
Application: IHC ; IF ; Ip ; WB
Reactivity: Human ; Mouse ; Pig
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Irene Leigh
Institute: Queen Mary University of London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Keratin14 [LL001]
- Research fields: Cell signaling and signal transduction
- Clone: LL001
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 50 kDa
- Strain: Balb/c
- Reactivity: Human ; Mouse ; Pig
- Host: Mouse
- Application: IHC ; IF ; Ip ; WB
- Description: Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types.
- Immunogen: Peptide (last 16 carboxy-terminal amino acids of human keratin) coupled to thyroglobulin by the extra Cys added at the N terminus.
- Isotype: IgG2a kappa
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Tonsil or lymph node
Target Details
- Target: Keratin 14
- Molecular weight: 50 kDa
- Tissue cell line specificity: Tonsil or lymph node
- Target background: Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types.
Applications
- Application: IHC ; IF ; Ip ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Sun et al. 2011. Endocrinology. 152(8):3233-45. PMID: 21628386.
- Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
- Karashima et al. 2002. J Cell Sci. 115(Pt 24):5027-37. PMID: 12432088.
- Interaction of periplakin and envoplakin with intermediate filaments.
- D'Alessandro et al. 2002. J Cell Sci. 115(Pt 22):4341-51. PMID: 12376565.
- Keratin mutations of epidermolysis bullosa simplex alter the kinetics of stress response to osmotic shock.
- Purkis et al. 1990. J Cell Sci. 97 ( Pt 1):39-50. PMID: 1701769.
- Antibody markers of basal cells in complex epithelia.
- Leigh et al. 1988. J Invest Dermatol. 90(5):639-42. PMID: 3283248.
- Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
