Anti-Keratin 5/6/18 [LP34]

Monoclonal antibody with broad reactivity against human keratins 5,6 and 18.

Contributors

Inventor Institute
Birgit Lane Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151125
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cell signaling and signal transduction
Application: ELISA ; IHC ; IF ; WB
Target: Keratin 5/6/18 (pan-epithelial)
Reactivity: Human
Clone: LP34
Host: Mouse
Class: Monoclonal
Product description: Monoclonal antibody with broad reactivity against human keratins 5,6 and 18.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 10 kDa
Immunogen: Detergent-insoluble fraction of psoriatic human epidermis
Immunogen Uniprot ID: P05783
Myeloma used: Sp2/0-Ag14
Target background: Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. Keratins demonstrate tissue and differentiation specific expression profiles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is member of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. These cytokeratins have been reported to be expressed in tumour cells of epithelial origin and less commonly of mesothelial origin, however non-epithelial tumours, e.g. lymphomas, do not express these cytokeratins. Anti-keratin 5/6/18 (LP34) shows a broad pattern of reactivity with human epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labelled whether they are ectodermal, mesodermal, or endodermal in origin. However, the recognition of cytokeratin 18 on paraffin sections using this antibody may be variable.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Barton et al. 2009. Neurosci Lett. 455(1):60-4. PMID: 19429107.

ELP3 localises to mitochondria and actin-rich domains at edges of HeLa cells.

Gardiner et al. 2008. Neuroscientist. 14(3):240-50. PMID: 18270312.

Defects in tongue papillae and taste sensation indicate a problem with neurotrophic support in various neurological diseases.

Karashima et al. 2002. J Cell Sci. 115(Pt 24):5027-37. PMID: 12432088.

Interaction of periplakin and envoplakin with intermediate filaments.

Braun-Falco et al. 1999. Gene Ther. 6(3):432-41. PMID: 10435093.

Efficient gene transfer into human keratinocytes with recombinant adeno-associated virus vectors.

Taylor-Papadimitriou et al., 1987. Keratin expression in the mammary gland. In, The Mammary Gland: Development, Regulation and Function, M.C. Neville and C. Daniel (Eds). Plenum Pub. Corp. 1987, 181-215.

Taylor-Papadimitriou et al., 1983. Cell lineages and interactions in neoplastic expression in the human breast. In, "Understanding Breast Cancer: Clinical and Laboratory Concepts". Eds. Rich, M., Hager, J.C. and Furmanski, P. Marcel Dekker Inc., New Yo

Related Tools for Antibodies

Cat. # Tool Name
151030 Anti-Progesterone [11P14] Key Info View Tool
151015 Anti-CyclinA [E70.1] Key Info View Tool
151023 Anti-IL12 [1-1D5] Key Info View Tool
151024 Anti-Hairy [1/24] Key Info View Tool
151039 Anti-Integrin aVb3 [23C6] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.