Cat. #161653
Anti-integrin a2 [P4B4]
Cat. #: 161653
Sub-type: Primary antibody
Target: Integrin alpha 2
Class: Monoclonal
Application: Western Blotting, Immunoprecipitation, immunofluorescence
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elizabeth A. Wayner
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-integrin a2 [P4B4]
- Research fields: Cell biology
- Clone: P4B4
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: 150kDa
- Reactivity: Human
- Host: Mouse
- Application: Western Blotting, Immunoprecipitation, immunofluorescence
- Description: Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin ?2 (P4B4) is a mouse monoclonal antibody raised against HT-1080. MoAb developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native native collagen. P4B4 had an hinhibitory effect on cell adhesion.
- Immunogen: HT1080 human fibrosarcoma cell line
- Isotype: IgG1
Target Details
- Target: Integrin alpha 2
- Molecular weight: 150kDa
- Target background: CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Antibody bound and inhibited cell adhesion to type VI and I collagens (and bound type III, IV, and V collagens with no inhibition) only and not to fibronectin or laminin
Applications
- Application: Western Blotting, Immunoprecipitation, immunofluorescence
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required
References
- Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
- Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064
