Cat. #161666
Anti-HLA-G [87G]
Cat. #: 161666
Sub-type: Primary antibody
Availability: 3-4 weeks
Target: HLA-G
Class: Monoclonal
Application: FACS, IP(weak), ELISA, Histology. No western
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Daniel Geraghty
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: lshitani et al.2003. Journal of lmmunology. 171(3):1376-84. PMID: 12874228
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-HLA-G [87G]
- Cancer: Blood cancer;Breast cancer;Genitourinary cancer;Skin cancer
- Research fields: Developmental biology;Immunology
- Clone: 87G
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: The heavy chain is approximately 45 kDa
- Reactivity: Human
- Host: Mouse
- Application: FACS, IP(weak), ELISA, Histology. No western
- Description: HLA-G has long been assumed a major factor in this immune accommodation because of its localized expression and because of low levels of allelic polymorphism. This MoAb was developed to examine the role of all three nonclassical class I Ags in the maternal immune response during pregnancy. MoAb 87G recognizes recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses.
- Isotype: IgG2a,?
Target Details
- Target: HLA-G
- Molecular weight: The heavy chain is approximately 45 kDa
- Target background: HLA-G are expressed in the placenta, a membrane form and a soluble form, the latter containing an altered carboxyl terminu. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (?2 microglobulin). The heavy chain is anchored in the membrane.
Applications
- Application: FACS, IP(weak), ELISA, Histology. No western
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage buffer: PBS, pH 7.2
- Storage conditions: 4° C
- Shipping conditions: Dry ice
References
- lshitani et al.2003.Journal of lmmunology. 171(3):1376-84.PMID: 12874228
- Lee et al.1995. Immunity.(5):591-600.PMID: 7584149
