FIH, Factor Inhibiting HIF1 (Hypoxia-Inducible Factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia.
| Inventor | Institute |
|---|---|
| Helen Turley | University of Oxford |
| Cat. #: | 151316 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Genetics |
| Application: | IHC ; IF ; WB |
| Target: | Factor Inhibiting HIF1 (FIH) |
| Reactivity: | Human |
| Clone: | FIH162C |
| Host: | Mouse |
| Class: | Monoclonal |
| Product description: | FIH, Factor Inhibiting HIF1 (Hypoxia-Inducible Factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia. |
|---|---|
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 40 kDa |
| Immunogen: | Full length human FIH expressed in Escherichia coli BL21(DE3) cells. |
| Myeloma used: | P3/NS1/1-Ag4.1 |
| Target background: | FIH, Factor Inhibiting HIF1 (Hypoxia-Inducible Factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia. |
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| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Wollenick et al. 2012. Nucleic Acids Res. 40(5):1928-43. PMID: 22075993. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Yan et al. 2009. Br J Cancer. 101(7):1168-74. PMID: 19724277. BRCA1 tumours correlate with a HIF-1alpha phenotype and have a poor prognosis through modulation of hydroxylase enzyme profile expression. Cockman et al. 2006. Proc Natl Acad Sci U S A. 103(40):14767-72. PMID: 17003112. Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected]. |
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