Anti-dHomer [2F7]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-dHomer [2F7]
• Research fields: Neurobiology
• Clone: 2F7
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Drosophila
• Host: Mouse
• Application: IF
• Description: dHomer (dHom), the Drosophila homolog of the vertebrate Homer is an F-actin binding protein. In mouse brain, Homer1a protein binds to the group I metabotropic glutamate receptors and competes against other constitutively expressed Homer-related proteins to regulate synaptic metabotropic glutamate receptors properties. Homer proteins are bipartite, consisting of an N-terminal class II Enabled/Vasp homology (EVH1) domain (Barzik et al., 2001) and a C-terminal coiled-coil (CC) domain that mediates self-association (Brakeman et al., 1997; Tu et al., 1998; Xiao et al., 2000). The EVH1 domain binds to F-actin, proline-rich sequences in group I metabotropic glutamate receptors, inositol trisphosphate receptors, ryanodine receptors, and Shank family proteins. Homer proteins also self associate and function as adaptors to couple interacting proteins implicated in synaptogenesis, signal transduction, receptor trafficking, and axon pathfinding. In mammals, there are three independent homer genes identified and at least six Homer proteins are formed due to alternative splicing. (Xiao et al., 1998; Kato et al., 1998). However, there is only one homer-related gene in the Drosophila genome. The function of Drosophila homer (d-hom) has been implicated in the control of locomotor activity and behavioral plasticity (Diagana et al., 2002).
• Immunogen: GSTd Homer (full-length) fusion protein
• Isotype: IgG1 kappa
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Drosophila embryos

Target Details

• Target: Drosophila homolog of the vertebrate Homer, an F-actin binding protein
• Target background: dHomer (dHom), the Drosophila homolog of the vertebrate Homer is an F-actin binding protein. In mouse brain, Homer1a protein binds to the group I metabotropic glutamate receptors and competes against other constitutively expressed Homer-related proteins to regulate synaptic metabotropic glutamate receptors properties. Homer proteins are bipartite, consisting of an N-terminal class II Enabled/Vasp homology (EVH1) domain (Barzik et al., 2001) and a C-terminal coiled-coil (CC) domain that mediates self-association (Brakeman et al., 1997; Tu et al., 1998; Xiao et al., 2000). The EVH1 domain binds to F-actin, proline-rich sequences in group I metabotropic glutamate receptors, inositol trisphosphate receptors, ryanodine receptors, and Shank family proteins. Homer proteins also self associate and function as adaptors to couple interacting proteins implicated in synaptogenesis, signal transduction, receptor trafficking, and axon pathfinding. In mammals, there are three independent homer genes identified and at least six Homer proteins are formed due to alternative splicing. (Xiao et al., 1998; Kato et al., 1998). However, there is only one homer-related gene in the Drosophila genome. The function of Drosophila homer (d-hom) has been implicated in the control of locomotor activity and behavioral plasticity (Diagana et al., 2002).

Applications

• Application: IF

Handling

• Format: Liquid
• Concentration: 1mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C