Cat. #151794
Anti-Desmoglein [33-3D]
Cat. #: 151794
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Desmoglein 1 and 2
Class: Monoclonal
Application: EM (EM) ; IHC ; IF ; WB
Reactivity: Bovine ; Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: University of Southampton
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Desmoglein [33-3D]
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction
- Clone: 33-3D
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Bovine ; Human
- Host: Mouse
- Application: EM (EM) ; IHC ; IF ; WB
- Description: 33-3D is directed against desmoglein in mammalian tissues, tumours and cultured cells.
- Immunogen: Bovine desmoglein (desmosomal glycoprotein 1) from nasal epidermis
- Isotype: IgM
- Myeloma used: P3/NS1/1-Ag4.1
- Recommended controls: 33-3D was validated on MDCK cells which are fixed using methanol (Vilela et al 1995).
Target Details
- Target: Desmoglein 1 and 2
- Tissue cell line specificity: 33-3D was validated on MDCK cells which are fixed using methanol (Vilela et al 1995).
- Target background: Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis.
Applications
- Application: EM (EM) ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Krunic et al. 1998. Br J Cancer. 77(8):1275-9. PMID: 9579833.
- Immunohistochemical staining for desmogleins 1 and 2 in keratinocytic neoplasms with squamous phenotype: actinic keratosis, keratoacanthoma and squamous cell carcinoma of the skin.
- Krunic et al. 1996. Acta Derm Venereol. 76(5):394-8. PMID: 8891017.
- Differential expression of desmosomal glycoproteins in keratoacanthoma and squamous cell carcinoma of the skin: an immunohistochemical aid to diagnosis.
- SchĤfer et al. 1996. Differentiation. 60(2):99-108. PMID: 8641550.
- Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues.
- Vilela et al. 1995. J Cell Sci. 108 ( Pt 4):1743-50. PMID: 7615689.
- A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
- Collins et al. 1990. Br J Cancer. 62(5):796-805. PMID: 2123114.
- A study of desmosomes in colorectal carcinoma.
- Vilela et al. 1987. J Pathol. 153(4):365-75. PMID: 3323436.
- Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.
