Cat. #151720
Anti-Desmoglein 1 [32-2B]
Cat. #: 151720
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Desmoglein 1
Class: Monoclonal
Application: EM (EM) ; IHC ; IF ; WB
Reactivity: Bovine ; Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Garrod
Institute: University of Southampton
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Desmoglein 1 [32-2B]
- Alternate name: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction
- Clone: 32-2B
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Bovine ; Human
- Host: Mouse
- Application: EM (EM) ; IHC ; IF ; WB
- Description: Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.
- Immunogen: Bovine desmoglein (desmosomal glycoprotein I) from nasal epidermis
- Isotype: IgG2a kappa
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: Desmoglein 1
- Target background: Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.
Applications
- Application: EM (EM) ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Maruani et al. 2008. Am J Clin Pathol. 130(3):369-74. PMID: 18701409.
- Immunostaining with antibodies to desmoglein provides the diagnosis of drug-induced pemphigus and allows prediction of outcome.
- A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
- Vilela et al. 1995. J Cell Sci. 108 ( Pt 4):1743-50. PMID: 7615689.
- Harada et al. 1992. Int J Oral Maxillofac Surg. 21(6):346-9. PMID: 1282923.
- Immunohistochemical detection of desmosomes in oral squamous cell carcinomas: correlation with differentiation, mode of invasion, and metastatic potential.
- Conn et al. 1990. Br J Urol. 65(2):176-80. PMID: 2317652.
- Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas.
- Vilela et al. 1987. J Pathol. 153(4):365-75. PMID: 3323436.
- Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.
