LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB.
| Inventor | Institute |
|---|---|
| Irene Leigh | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 152691 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Cell biology |
| Application: | ELISA ; IHC ; IF ; WB |
| Target: | Collagen Type VII |
| Reactivity: | Human |
| Clone: | LH7.2 |
| Host: | Mouse |
| Class: | Monoclonal |
| Product description: | Type VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB. |
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| Conjugation: | Unconjugated |
| Isotype: | IgG1 kappa |
| Molecular weight: | 250 kDa |
| Immunogen: | Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions. The epitope is on the NC1 domain. |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | Type VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB. |
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| Format: | Liquid |
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| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Eldardiri et al. 2012. Tissue Eng Part A. 18(5-6):587-97. PMID: 21939396. Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of wound repair and regeneration. Al-Refu et al. 2011. Clin Exp Dermatol. 36(1):63-8. PMID: 20637030. Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus. Watson et al. 2001. J Invest Dermatol. 116(5):672-8. PMID: 11348454. A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents. Shimizu et al. 1990. Br J Dermatol. 122(5):577-85. PMID: 2354110. Epidermolysis bullosa acquisita antigen and the carboxy terminus of type VII collagen have a common immunolocalization to anchoring fibrils and lamina densa of basement membrane. |
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