Cat. #151913
Anti-CLEC2 [AYP1]
Cat. #: 151913
Unit size: 100 ug
Availability: 1-2 weeks
Target: C-type lectin-like receptor 2, CLEC1b
Class: Monoclonal
Application: FACS ; IF ; IP ; Fn
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Steve Watson
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CLEC2 [AYP1]
- Research fields: Immunology;Tissue-specific biology
- Clone: AYP1
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 26 kDa
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IF ; IP ; Fn
- Description: Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
- Immunogen: A recombinant extracellular fragment of human CLEC-2 (aa 68-229), which is the extracellular domain of the protein
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Platelets
Target Details
- Target: C-type lectin-like receptor 2, CLEC1b
- Molecular weight: 26 kDa
- Target background: Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
Applications
- Application: FACS ; IF ; IP ; Fn
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: Hybridomas were cultured at 37? C and 96% relative humidity in air containing 6% CO2. Cells were grown in complete DMEM + Pen/strep + FCS was used at 10%. Cell density: Cells should be kept between 1-2x105 cells/ml and 5x105 cells/ml.
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-CLEC2 [AYP2] ; CLEC2 Floxed KO Mouse ; Anti-CLEC2 [17D9]
References
- Gitz E et al, Blood, 2014. PMID: 25150298
