Anti-CD45 [C363.16A]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD45 [C363.16A]
• Alternate name: YCD45R-1
• Research fields: Immunology
• Tool sub type: Primary antibody
• Class: Monoclonal
• Purpose: Marker
• Conjugation: Unconjugated
• Molecular weight: 190 kDa and 220 kDa
• Reactivity: Mouse
• Host: Rat
• Application: FACS ; IF ; IP ; WB
• Description: Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, na?ve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a criti...
• Immunogen: cloned T cell lines belonging to the helper T cell (Th2) subset, final boost was given with helper/Th2 clone D10
• Immunogen uniprot id: P08575
• Isotype: IgG

Target Details

• Target: CD45
• Molecular weight: 190 kDa and 220 kDa
• Target background: Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, nave T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a cr...

Applications

• Application: FACS ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

• Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.
• Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.
• Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.
• Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.
• Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.
• Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.
• Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.