#155241

Anti-CD45 [C363.16A]

Cat. #155241

Anti-CD45 [C363.16A]

Cat. #: 155241

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: CD45

Class: Monoclonal

Application: FACS ; IF ; IP ; WB

Reactivity: Mouse

Host: Rat

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Yale University

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CD45 [C363.16A]
  • Alternate name: YCD45R-1
  • Research fields: Immunology
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Purpose: Marker
  • Conjugation: Unconjugated
  • Molecular weight: 190 kDa and 220 kDa
  • Reactivity: Mouse
  • Host: Rat
  • Application: FACS ; IF ; IP ; WB
  • Description: Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, na?ve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a criti...
  • Immunogen: cloned T cell lines belonging to the helper T cell (Th2) subset, final boost was given with helper/Th2 clone D10
  • Immunogen uniprot id: P08575
  • Isotype: IgG

Target Details

  • Target: CD45
  • Molecular weight: 190 kDa and 220 kDa
  • Target background: Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, nave T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a cr...

Applications

  • Application: FACS ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.
  • Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.
  • Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.
  • Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.
  • Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.
  • Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.
  • Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.