Cat. #161647
Anti-CD34 [1H6]
Cat. #: 161647
Sub-type: Primary antibody
Target: CD34
Class: Monoclonal
Application: Western blotting, flow cytometry, CFU-GM assay, LTC-IC assay, ELISA
Reactivity: Human
Host: Canine
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Richard Nash
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: McSweeney et al. 1998.Blood. 91(6):1977-86. PMID: 9490680
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD34 [1H6]
- Research fields: Stem cell biology
- Clone: 1H6
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: 90kDa
- Reactivity: Human
- Host: Canine
- Application: Western blotting, flow cytometry, CFU-GM assay, LTC-IC assay, ELISA
- Description: Good animal models in which to refin studies of CD34 cell fractions are limited. However, this MoAb detected 4% to 17% CD34 cells in murine Bone Marrow(BM) cells, somewhat higher than the 1% to 5% CD34 cells detected in human BM.
- Immunogen: A recombinant CD34 murine Ig fusion molecule (CD34-Ig)
- Isotype: IgG-1
Target Details
- Target: CD34
- Molecular weight: 90kDa
- Target background: CD34 has previously been define as a marker of hematopoietic progenitors and is expressed on canine bone marrow (BM) progenitor cells in a manner analogous to that found in humans.
Applications
- Application: Western blotting, flow cytometry, CFU-GM assay, LTC-IC assay, ELISA
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
References
- McSweeney et al. 1998.Blood. 91(6):1977-86.PMID:PMID: 9490680
- Wayner et al. 1987.J Cell Biol.105 (1987): 1873.https://doi.org/10.1083/jcb.105.4.1873
