Anti-BCL2 [bcl-2/124]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-BCL2 [bcl-2/124]
• Research fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism
• Clone: bcl-2/124
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Disease: Breast Cancer
• Application: IHC ; WB
• Description: Monoclonal antibody which binds anti-apoptotic regulator BCL2, used to aid in differentiating between various lymphomas and as prognostic biomarker in various cancers.
• Immunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
• Immunogen uniprot id: P10415
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Bcl2
• Target background: BCL2 is a member of the BCL-2 family of regulatory proteins, involved in apoptosis, which promotes cell survival and inhibits the role of pro-apoptotic proteins such as Bax and Bak. The pro-survival protein BCL2 derives its name from B-cell lymphoma 2. It is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. Damage to the Bcl-2 gene has been identified as a possible cause of schizophrenia and autoimmunity, a cause of cancers, including melanoma, breast, prostate, chronic lymphocytic leukaemia, and lung cancer. It also provides resistance to cancer treatments . This antibody was created to see if the BCL2 protein is a specific marker to the 14;18 chromosomal translocation in human follicular lymphoma. Previous studies claimed the BCL2 protein was not found in normal tissue but was present in all lymphomas where this specific translocation was observed. This antibody helped show the immunohistologic demonstration of BCL2 isn't specific for the presence of 14;18 translocation.

Applications

• Application: IHC ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

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