pGEX4T1-CBL-B Vector

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: pGEX4T1-CBL-B Vector
• Alternate name: C-CBL, CBL-B, CBL-C, C-CBL2, FRA11B, NSLL, RNF55, Cbl proto-oncogene, Casitas B-lineage Lymphoma
• Research fields: Cancer;Cell signaling and signal transduction;Genetics
• Tool sub type: pGEX4T1
• Backbone size without insert: 4969
• Bacterial resistance: Ampicillin
• Description: Over 600 E3 ligases have been identified in mammals and most are part of the RING family of E3 ligases. The monomeric family of CBL RING E3 ligases (c-CBL, CBL-B and CBL-C) attenuate nonreceptor and receptor tyrosine kinase signalling through ubiquitination and direct the receptor tyrosine kinases for degradation through the endocytic or proteasomal pathways. Members of the CBL family share a highly conserved N terminus comprising of a tyrosine kinase binding domain, a linker helix region and a RING domain. The more variable C terminus possessing a proline-rich region and a string of terminal amino acids or 'extension'. Mutations in this gene have been implicated in several human cancers including acute myeloid leukaemia. The protein CBL is a ubiquitously expressed 982 amino acid E3 ubiquitin-protein ligase involved in cell signalling and protein ubiquitination.

Handling

• Storage conditions: -20° C
• Shipping conditions: Dry Ice

Target Details

• Target: CBL-B fragment encompassing the TKBD, LHR and RING domain: residues 36–427

Related Tools

• Related tools: pRSF-1b-UbcH5B Vector

References

• Dou et al. 2013. Nat Struct Mol Biol. 20(8):982-6. PMID: 23851457.
• Essentiality of a non-RING element in priming donor ubiquitin for catalysis by a monomeric E3.