#153815

MSTI Fluorescence High-Throughput Drug Assay

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Cat. #153815

MSTI Fluorescence High-Throughput Drug Assay

Cat. #: 153815

Sub-type: Fluorescent Probe

Availability: Please enquire for quantities and pricing

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: University of Wisconsin-Milwaukee

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: MSTI Fluorescence High-Throughput Drug Assay
  • Research fields: Drug development
  • Molecular formula: C19H20NS
  • Tool sub type: Fluorescent Probe
  • Description: This novel fluorescence-based high throughput assay allows for the detection of thiol-reactive drug candidates that are likely to irreversibly interact with biological targets. These promiscuous inhibitors can be identified rapidly, in parallel, for small molecule screening libraries using 384 or 1536 well plate formats. Testing small molecules for their ability to modulate cysteine residues of proteins in the early stages of drug discovery is expected to increase efficiency and success of ev...
  • Iupac: Acetyl-(E)-2-(4-mercaptostyryl)-1,3,3-trimethyl-3H-indol-1-ium
  • Molecular weight: 294.1
  • Solubility: DMSO, DMF, methanol, water (partially)
  • Additional notes: ▪More accurate – Less interference from molecules due to use of far red spectrum▪ Faster – Can be used for high throughput screening (1536-well plate format)▪ More versatile – Identifies both electrophilic and redox reactive compounds▪ Stable product – Use of acetylated precursor allows for storage of the assay probe and itsreliable generation in situ

Handling

  • Storage conditions: -20° C, protect from light

References

  • McCallum et al. 2013. J Biomol Screen. 18(6):705-13. PMID: 23446699.
  • High-throughput identification of promiscuous inhibitors from screening libraries with the use of a thiol-containing fluorescent probe.