#153336

ROCK2 Floxed Mouse

Cat. #153336

ROCK2 Floxed Mouse

Cat. #: 153336

Sub-type: Mouse

Availability: 6-8 weeks

Model: Transgenic

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Contributor

Inventor: Chris Marshall

Institute: The Institute of Cancer Research

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: ROCK2 Floxed Mouse
  • Alternate name: ROCK2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; ROCK-II
  • Research fields: Cancer;Cell biology;Developmental biology
  • Tool sub type: Mouse
  • Model: Transgenic
  • Conditional: Yes
  • Conditional description: Requires expression of Cre recombinase to mediate excision of the lox P sites generating a non-functional ROCK 2 protein
  • Description: Rho associated coiled-coil containing protein kinases (ROCKs) exist in mammals, zebrafish, Xenopus, C. elegans and Drosophila. They are mainly involved in regulating the shape and movement of cells through acting on the cytoskeleton. Two mouse ROCK isoforms ROCK1 and ROCK2 have been identified. ROCK1 is mainly expressed in the lung, liver, spleen, kidney and testis and ROCK2 is distributed mostly in the brain and heart. ROCK1 and ROCK2 are downstream effectors of the Rho subfamily of small GTPases, activated by Rho GTPases. ROCK2, an isoenzyme of ROCK1, is a serine/threonine kinase and regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibres and activation of the c-fos serum response element. ROCK1 is involved in cancer initiation and progression presumably through mechanisms related to metastasis, cell motility and angiogenesis.
  • Genetic background: Lox P sequences were inserted to flank exons 5 and 6 of the ROCK 2 gene
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Production details: Lox P sequences were inserted to flank exons 5 and 6 of the ROCK 2 gene
  • Breeding information: Mice are viable and have a normal lifespan

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Rho Associated Coiled-Coil Containing Protein Kinase 2

Related Tools

  • Related tools: ROCK1 Floxed Mouse

References

  • Kmper et al. 2016. Elife. 5:e12994. PMID: 26765561.
  • Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis.