Cat. #158395
Pik3ca-H1047R conditional Flp inducible
Cat. #: 158395
Sub-type: Mouse
Availability: 8-10 weeks
Model: Conditional KO
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Bart Vanhaesebroeck
Institute: Ludwig Institute for Cancer Research
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Pik3ca-H1047R conditional Flp inducible
- Research fields: Cancer
- Tool sub type: Mouse
- Model: Conditional KO
- Conditional: Yes
- Genetic background: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110akinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Phenotype: Homozygous and heterozygous mice are phenotypically normal.
- Production details: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110Îąkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Additional notes: Homozygous and heterozygous mice are phenotypically normal.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: PIK3CA
References
- Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.
