p110beta PI3K-D931A (129)

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: p110beta PI3K-D931A (129)
• Research fields: Cancer
• Tool sub type: Mouse
• Model: Knock-In
• Application: RNAseq, histological studies and hormone dosage.
• Genetic background: Knock-in mice in which the endogenous PIK3CB/p110beta PI3K gene is mutated so that it now encodes a p110beta protein with the D931A mutation in the ATP binding site, converting it to a kinase-dead p110beta protein which is expressed at the same level as wild-type p110beta. These mice have been backcrossed onto the sv129 background.
• Phenotype: Mice show prenatal partial lethality at at different embryonic stages (see PMID 26132308 for details). Homozygous male mice that are born are infertile with smaller than normal testicles. They cannot produce offspring.
• Production details: Knock-in mice in which the endogenous PIK3CB/p110beta PI3K gene is mutated so that it now encodes a p110beta protein with the D931A mutation in the ATP binding site, converting it to a kinase-dead p110beta protein which is expressed at the same level as wild-type p110beta. These mice have been backcrossed onto the sv129 background.
• Additional notes: Mice show prenatal partial lethality at at different embryonic stages (see PMID 26132308 for details). Homozygous male mice that are born are infertile with smaller than normal testicles. They cannot produce offspring.

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: PIK3CB

Application Details

• Application: RNAseq, histological studies and hormone dosage.

References

• Guillermet-Guibert et al. 2015. PLoS Genet. 11(7):e1005304. PMID: 26132308.
• Kulkarni et al. 2011. Sci Signal. 4(168):ra23. PMID: 21487106.