Cat. #153173
Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse
Cat. #: 153173
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Glioma
Model: Knock-In
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ian Tomlinson
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse
- Alternate name: IDH1, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
- Research fields: Cancer;Neurobiology
- Tool sub type: Mouse
- Disease: Glioma
- Model: Knock-In
- Conditional: Yes
- Description: Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.
- Phenotype: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen.
- Strain: C57BL/6
- Additional notes: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen. The mice carry a tamoxifen-inducible Cre, Nestin-CreER(T2) which in adult mice targets Cre to the subventricular zone (SVZ) and the other major neurogenic niche, the subgranular zone (SGZ) of the hippocampal dentate gyrus. When induced using tamoxifen to express the Idh1R132H mutant protein the mice are referred to as Tam-Idh1-KI mice.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Isocitrate dehydrogenase 1
References
- Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.
- Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.
