#153173

Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse

Cat. #153173

Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse

Cat. #: 153173

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Glioma

Model: Knock-In

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Contributor

Inventor: Ian Tomlinson

Institute: University of Oxford

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse
  • Alternate name: IDH1, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
  • Research fields: Cancer;Neurobiology
  • Tool sub type: Mouse
  • Disease: Glioma
  • Model: Knock-In
  • Conditional: Yes
  • Description: Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.
  • Phenotype: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen.
  • Strain: C57BL/6
  • Additional notes: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen. The mice carry a tamoxifen-inducible Cre, Nestin-CreER(T2) which in adult mice targets Cre to the subventricular zone (SVZ) and the other major neurogenic niche, the subgranular zone (SGZ) of the hippocampal dentate gyrus. When induced using tamoxifen to express the Idh1R132H mutant protein the mice are referred to as Tam-Idh1-KI mice.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Isocitrate dehydrogenase 1

References

  • Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.
  • Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.