Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse

Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.

Contributors

Inventor Institute
Ian Tomlinson University of Oxford
Cat. #: 153173
Research Fields: Cancer;Neurobiology
Target: Isocitrate dehydrogenase 1
disease: Glioma
Model: Knock-In
Strain: C57BL/6
Phenotype: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen.
Alternate name: IDH1, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Product description: Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.
Conditional: Yes
Additional notes: Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen. The mice carry a tamoxifen-inducible Cre, Nestin-CreER(T2) which in adult mice targets Cre to the subventricular zone (SVZ) and the other major neurogenic niche, the subgranular zone (SGZ) of the hippocampal dentate gyrus. When induced using tamoxifen to express the Idh1R132H mutant protein the mice are referred to as Tam-Idh1-KI mice.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.

Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.

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