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#151564

K14dNLef1 Mouse

Cat. #151564

K14dNLef1 Mouse

Cat. #: 151564

Sub-type: Mouse

Availability: 8-10 weeks

Disease: Cancer

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Fiona Watt

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: K14dNLef1 Mouse
  • Cancer: Gynaecologic cancer;Skin cancer
  • Cancers detailed: Sebaceous adenoma;Sebeoma;Squamous papilloma
  • Research fields: Cancer;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Conditional: Yes
  • Conditional description: Conditional expression of deltaN-Lef1 under Keratin 14 promoter giving tissue-specific epidermal expression.
  • Description: In vivo study of Lef1 activity in skin; model for hair growth & follicle formation; disease model for skin tumour formation (sebaceous adenoma, sebeoma, squamous papilloma)
  • Genetic background: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.
  • Phenotype: Tumourigenesis (skin); skin abnormalities (hair follicle, sebaceous gland)
  • Production details: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Lef1 N-terminal mutant (constituitive transcirptional activity)

References

  • Niemann et al. 2002. Development. 129(1):95-109. PMID: 11782404.
  • Expression of DeltaNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and formation of skin tumours.

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