Cat. #151564
K14dNLef1 Mouse
Cat. #: 151564
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Cancer
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Fiona Watt
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: K14dNLef1 Mouse
- Cancer: Gynaecologic cancer;Skin cancer
- Cancers detailed: Sebaceous adenoma;Sebeoma;Squamous papilloma
- Research fields: Cancer;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology
- Tool sub type: Mouse
- Disease: Cancer
- Conditional: Yes
- Conditional description: Conditional expression of deltaN-Lef1 under Keratin 14 promoter giving tissue-specific epidermal expression.
- Description: In vivo study of Lef1 activity in skin; model for hair growth & follicle formation; disease model for skin tumour formation (sebaceous adenoma, sebeoma, squamous papilloma)
- Genetic background: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.
- Phenotype: Tumourigenesis (skin); skin abnormalities (hair follicle, sebaceous gland)
- Production details: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Lef1 N-terminal mutant (constituitive transcirptional activity)
References
- Niemann et al. 2002. Development. 129(1):95-109. PMID: 11782404.
- Expression of DeltaNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and formation of skin tumours.
