Cat. #151562
JamC -/- Mouse
Cat. #: 151562
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Neuropathy
Model: Knock-Out
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ralf H. Adams
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: JamC -/- Mouse
- Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
- Tool sub type: Mouse
- Disease: Neuropathy
- Model: Knock-Out
- Conditional: No
- Description: In vivo study of JAM-C knockout; in vivo study of spermiogenesis; in vivo study of neuronal networks and integrity
- Genetic background: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
- Phenotype: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
- Zygosity: Heterozygous
- Production details: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
- Additional notes: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Junctional adhesion molecule-C (JAM-C)
References
- Gliki et al. 2004. Nature. 431(7006):320-4. PMID: 15372036.
- Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C.
