Cat. #151519
BRaf1 KI Mouse
Cat. #: 151519
Sub-type: Mouse
Availability: 6-8 weeks
Model: Knock-In
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: BRaf1 KI Mouse
- Alternate name: LSL B-RafV6E
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Colorectal;Melanoma
- Research fields: Cancer;Cell signaling and signal transduction;Genetics
- Tool sub type: Mouse
- Model: Knock-In
- Conditional: Yes
- Conditional description: B-Raf gene with loxP sites flanking wildtype exons 15 to 18 (plus STOP) inserted before mutant exons 15 (V600E) to 18, enabling conditional Cre-mediate excision of wildtype exons and simultaneous knockin of mutant exons.
- Description: Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways The LSL-B-Raf-V600E targeting vector, containing a loxP flanked minigene+polyA encoding wildtype exons 15 to 18, amd a loxP flanked resistance cassette, and a substitution of residue 600 (V to E) in the endogenous exon 15, was transfected into E14.1 ES cells. Properly targeted ES cells containing homologous recombination events were injected into C57BL6 balstocysts. Chimeric mice were backcrossed on the C57BL6 background.
- Genetic background: E14.1/C57BL6
- Zygosity: Heterozygous
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: BRaf V600E (oncogenic mutant)
References
- Mercer et al. 2005. Cancer Res. 65(24):11493-500. PMID: 16357158.
