ATF2 Flox Mouse

Conditional knockout of ATF2 DNA binding domain. Transcription factor (BZIP-type, AP1-family)

Contributors

Inventor Institute
Nic Jones Cancer Research UK, Manchester Institute
Cat. #: 151647
Research Fields: Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Immunology
Target: ATF2
Model: Conditional KO
Zygosity: Homozygous
Phenotype: Homozygous normal phenotype
Product description: Conditional knockout of ATF2 DNA binding domain. Transcription factor (BZIP-type, AP1-family)
Conditional: Yes
Genetic background: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library, cloned into pPGKneobpAlox2PGKDlacZ-pA-neo-loxP, transfected into 129/ola embryonic stem cells and selected for G418 resistance. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
Production details: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library, cloned into pPGKneobpAlox2PGKDlacZ-pA-neo-loxP, transfected into 129/ola embryonic stem cells and selected for G418 resistance. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Breitwieser et al. 2007. Genes Dev. 21(16):2069-82. PMID: 17699753.

Feedback regulation of p38 activity via ATF2 is essential for survival of embryonic liver cells.

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