Cat. #153454
VP Cell Line
Cat. #: 153454
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Skin
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Fiona Watt
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: VP Cell Line
- Alternate name: HPV, Human papillomavirus
- Cancer: Carcinoma;Gynaecologic cancer;Skin cancer
- Cancers detailed: Carincogenesis;Cervical;HPV;Skin cancer
- Research fields: Cancer;Cell biology;Drug development;Genetics;Immunology
- Tool sub type: Continuous
- Parental cell: Human epidermal keratinocytes - strain V
- Organism: Human
- Tissue: Skin
- Disease: Cancer
- Model: Immortalised Line
- Conditional: No
- Description: The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line
- Production details: Primary human keratinocytes from newborn foreskin samples were used as the source of cells. These cells were then grown in culture and at passage 2 transfected with the pSV2neo/16 plasmid which contains the entire HPV16 genome.
- Recommended controls: Human epidermal keratinocytes - strain V parental line
Target Details
- Target: HPV transfected human keratinocytes
Handling
- Format: Frozen
- Growth medium: Composed of a 1+3 mixture of Ham?Âs F12 and DMEM, supplemented with 1.8x10-4M adenine, 10 % FCS, 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ng/ml EGF and 10-10M cholera toxin. Cultures should be maintained at 37oC, and medium changed every 2-3 days
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: UP Cell Line
References
- Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.
- Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.
- Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.
- Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.
