UP Cell Line

The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line

Contributors

Inventor Institute
Fiona Watt Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 153455
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Carcinoma;Gynaecologic cancer;Skin cancer
Research Fields: Cancer;Cell biology;Drug development;Genetics;Immunology
Organism: Human
Tissue: Skin
Model: Immortalised Line
Cancer Types In Detail: Carincogenesis;Cervical;HPV;Skin cancer
Alternate name: HPV, Human papillomavirus
Product description: The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line
Conditional: No
Production details: Primary human keratinocytes from newborn foreskin samples were used as the source of cells. These cells were then grown in culture and at passage 2 transfected with the pSV2neo/16 plasmid which contains the entire HPV16 genome.
Parental cell line: Human epidermal keratinocytes – strain U
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Composed of a 1+3 mixture of Ham?‚Â’s F12 and DMEM, supplemented with 1.8×10-4M adenine, 10 % FCS, 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ng/ml EGF and 10-10M cholera toxin. Cultures should be maintained at 37?‚°C, and medium changed every 2-3 days.
Recommended controls: Human epidermal keratinocytes – strain U parental line
References:

Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.

Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.

Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.

Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.

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