UM-UC-9 (Human transitional cell carcinoma of the bladder) cell line

UM-UC-9 demonstrated efficient gene transduction via an adenoviral vector when compared to several of the other cells evaluated. UM-UC-9 also showed intermediate levels of Coxsackie adenovirus receptor (CAR) expression of the cells tested.

Contributors

Inventor Institute
H. Grossman ; Anita Sabichi University of Michigan
Cat. #: 160439
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Genitourinary cancer;Genitourinary cancer
Research Fields: Cancer;Cancer
Organism: Human
Tissue: Bladder
Gender: Male
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Human bladder transitional cell carcinoma;Human bladder transitional cell carcinoma
Alternate name: UM-UC-9; UMUC9; UC-9; University of Michigan-Urothelial Carcinoma-9
Product description: UM-UC-9 is a human transitional cell carcinoma cell line. This cell line is tumorigenic in athymic nude mice. UM-UC-9 demonstrated efficient gene transduction via an adenoviral vector when compared to several of the other cells evaluated. UM-UC-9 also showed intermediate levels of Coxsackie adenovirus receptor (CAR) expression of the cells tested. The UM-UC panel of urothelial carcinoma is a diverse range of 11 patient derived cell lines developed by the university of Michigan that have been well-categorised in the literature. These cells were assessed for their susceptibility to adenoviral mediated gene delivery, tumour growth in nude mice and differences in genetic alterations.
Gender: Male
CRISPR: Yes
Conditional: Yes
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells were grown in EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm2; using 0.05% trypsin/EDTA; 5% CO2, 37°C.
Mycoplasma free: Yes
References:

Sabichi et al. 2006. J Urol. 175(3 Pt 1):1133-7. PMID: 16469639.

Grossman et al. 1984. J Urol. 132(4):834-7. PMID: 6471236.

Citations And Reviews

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