#161902

TOV-3121EP

Cat. #161902

TOV-3121EP

Cat. #: 161902

Organism: Human

Tissue: Ovary

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne-Marie Mes-Masson and Diane Provencher

Institute: Centre Hospitalier de L’université de Montréal

Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: TOV-3121EP
  • Cancer: Gynaecologic cancer
  • Research fields: Cancer
  • Organism: Human
  • Tissue: Ovary
  • Donor: Donor was 62 years at age of diagnosis with stage 3 IIIC fallopian tube high-grade serous adnocarcinoma and a TP53 mutation
  • Disease: Cancer
  • Morphology: Formed spheroids with small, compact cores surrounded by loosely aggregated cells with an irregular margin
  • Growth properties: Mixed
  • Crispr: No
  • Description: Cell line derived from solid tumor of patient with Stage 3 IIC fallopian tube-HGSC and had received neo-adjuvant chemotherapy of carboplatin/taxol prior to specimen collection in 2006. Cell line had intermediate sensitivity to carboplatin and did not form SC or IP tumors in NRG mice.
  • Production details: Tissue was scraped into 100mm plate with complete OSE medium and maintained for 40 days with weekly culture medium replacement
  • Biosafety level: 1

Handling

  • Format: Frozen
  • Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
  • Temperature: 37° C
  • Atmosphere: 7% O2 and 5% CO2
  • Shipping conditions: Dry Ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
  • Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
  • Str profiling: TH01: 9.3 / D21S11: 30,32.2 / D5S818: 10,13 / D13S317: 12 / D7S820: 10 / D16S539: 12 / CSF1PO: 10,12 / vWA: 16,18 / TPOX: 8 / AMEL: X

References

  • Meng, et al. 2023. J Anim Sci. 3, 101:skad215. PMID: 37351870