Email ID
Cat. #161901
TOV-2881EP
Cat. #: 161901
Organism: Human
Tissue: Ovary
Disease: Cancer
£575.00
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Contributor
Inventor: Anne-Marie Mes-Masson and Diane Provencher
Institute: Centre Hospitalier de L’université de Montréal
Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519
Tool Details
*FOR RESEARCH USE ONLY
- Name: TOV-2881EP
- Cancer: Gynaecologic cancer
- Research fields: Cancer
- Organism: Human
- Tissue: Ovary
- Donor: Patient was 56 at time of diagnosis wiht stage 3 IIIC ovarian high-grade serous adenocarcinoma with TP53 mutation. Donor had no prior cancer hisotry themselves, but had a malignant neoplasm of the urinary tract
- Disease: Cancer
- Morphology: Did not form spheroids
- Growth properties: Mixed
- Crispr: No
- Description: Epithelial ovarian cancer cell line derived from solid tumors of patient who was diagnosted with Stage 3 IIIC ovarian high-grade serous adenocarcinoma had received neo-adjuvant chemotherapy of carboplatin/taxol prior to specimen collection in 2006. Cell line exhibited strong sensitivity to carboplatin. Cell line formed in vivo metastases and ascites when injected IP in mice, but did not form SC tumors. This cell line might be useful for studying disease spread at recurrence.
- Production details: Tissue was scraped into 100mm plate with complete OSE medium and maintained for 40 days with weekly culture medium replacement
- Biosafety level: 1
Handling
- Format: Frozen
- Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
- Temperature: 37° C
- Atmosphere: 7% O2 and 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
- Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
- Str profiling: TH01: 9 / D21S11: 32.2,33.2 / D5S818: 12 / D13S317: 9,11 / D7S820: 10,11 / D16S539: 12 / CSF1PO: 11 / vWA: 14,16 / TPOX: 11 / AMEL: X
References
- Guo, et al. 2022. Front Genet. 25, 13:844800. PMID: 35401671
- Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519
