Cat. #162196
SW480 EGFP-lamin A cell line clone 2c3
Cat. #: 162196
Organism: Human
Tissue: Colon
Model: Genetically modified cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stefan Przyborski
Institute: University of Durham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SW480 EGFP-lamin A cell line clone 2c3
- Alternate name: SW480 GFP-lamin clone 2c3
- Parental cell: SW480
- Clone: clone 2c3
- Organism: Human
- Tissue: Colon
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Genetically modified cell line
- Receptors of note: No
- Description: Colon cancer cell line with low endogenous lamin A protein expression, rescued by stable transfection of EGFP-lamin A. Suitable for studying the effect of the presence of lamin A on cancer cell properties.
- Recommended controls: Same cell line stably transfected with EGFP not tagged to any other DNA
Target Details
- Target: Lamin A
- Target background: Type V intermediate filament protein which forms part of the nuclear lamina. Primarily performs a structural function, but can also affect gene transcription.
Handling
- Format: Frozen
- Passage number: Not known. Cells are high passage (>100) due to being a cancer cell line from a culture bank and then going through multiple passages to grow up the stable transfectants.
- Growth medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin
- Atmosphere: Without CO2
- Shipping conditions: Dry ice
- Storage medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO
- Storage conditions: Liquid Nitrogen
- Initial handling information: Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media.
- Subculture routine: Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4.
- Cultured in antibiotics: penicillin/streptomycin
- Characterisation tests: RT-PCR, IF and Western Blotting to confirm expression of lamin A
- Str profiling: Unpublished WB analysis available
References
- Unpublished
