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#152434

SW1222 Cell Line

Cat. #152434

SW1222 Cell Line

Cat. #: 152434

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Colon

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: University of Oxford

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: SW1222 Cell Line
  • Cancer: Digestive / Gastrointestinal cancer
  • Cancers detailed: Human colorectal adenocarcinoma
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Growth mode: adherent
  • Conditional: Yes
  • Description: The SW1222 cell line was established from a patient with a moderately well differentiated adenocarcinoma of the colon classified as Dukes' stage C. SW1222 cells produce tumours in female athymic nu/nu nude mice.
  • Additional notes: STR-PCR Data: Amelogenin: X,Y CSF1PO: 11 D13S317: 9,13 D16S539: 9,10 D5S818: 9 D7S820: 10,12 THO1: 7 TPOX: 10 vWA: 18
  • Cellosaurus id: CVCL_3886

Applications

  • Application notes: STR-PCR Data: Amelogenin: X,Y CSF1PO: 11 D13S317: 9,13 D16S539: 9,10 D5S818: 9 D7S820: 10,12 THO1: 7 TPOX: 10 vWA: 18

Handling

  • Format: Frozen
  • Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

References

  • Bacac et al. 2016. Clin Cancer Res. 22(13):3286-97. PMID: 26861458.
  • Hsia et al. 2016. Proc Natl Acad Sci U S A. :. PMID: 27036009.
  • Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers.
  • A Novel Carcinoembryonic Antigen T-Cell Bispecific Antibody (CEA TCB) for the Treatment of Solid Tumors.
  • Browning et al. 1993. J Immunother Emphasis Tumor Immunol. 14(3):163-8. PMID: 8297898.
  • Loss of human leukocyte antigen expression ...

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