SK-MEL-28 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: SK-MEL-28 Cell Line
• Cancer: Skin cancer
• Organism: Human
• Gender: Male
• Tissue: Skin
• Donor: This cell line was established from an axillary lymph node of a 51-year-old male of unknown ethnicity.
• Morphology: Polygonal
• Growth properties: Adherent
• Model: Tumourigenic
• Model description: Tumourigenic in nude mice; forms malignant melanoma (large round cell type)
• Products or characteristics of interest: Karyotype: modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell. Isoenzymes: AK-1, 1-2; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1
• Description: SK-MEL-28 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras. It is also able to form tumours in nude mice.
• Application: 3D cell culture; High-throughput screening; Toxicology
• Biosafety level: 1

Target Details

• Target: Antigen expression: Blood Type A; Rh+; HLA A11, A26, B40, DRw4

Applications

• Application: 3D cell culture; High-throughput screening; Toxicology

Handling

• Growth medium: EMEM supplemented with FBS to a final concentration of 10%
• Temperature: 37° C
• Atmosphere: 5% CO2 in air
• Shipping conditions: Dry Ice
• Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
• Storage conditions: Vapor phase of liquid nitrogen
• Subculture routine: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking
• Str profiling: D3S1358: 16,18TH01: 7D21S11: 28,29D18S51: 12,16Penta_E: 8,12D5S818: 11,13D13S317: 11,12D7S820: 9.3,10D16S539: 9,12CSF1PO: 10,12Penta_D: 9,10Amelogenin: X,YvWA: 16,19D8S1179: 13TPOX: 8,12FGA: 19D19S433: 14D2S1338: 18

References

• Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.
• Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.