Cat. #161912
SK-LMS-01 Cell Line
Cat. #: 161912
Availability: 8-10 weeks
Organism: Human
Tissue: Vulva
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh J., et al. 1975. Springer; New York.115-159.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-LMS-01 Cell Line
- Cancer: Gynaecologic cancer;Sarcoma
- Organism: Human
- Gender: Female
- Tissue: Vulva
- Donor: 43-year-old, White female patient with leiomyosarcoma
- Morphology: Fibroblast
- Growth properties: Adherent
- Products or characteristics of interest: The cell line is aneuploid human female, with chromosome counts in the triploid to hypertriploid range. Neither of the X chromosomes are normal, but their q arms are detected in a translocation with chromosome N4. Normal chromosomes N1, N4, N13, and N17 are clearly under-represented, while chromosomes N6, N18, and N20 are consistently over-represented with respect to the copy numbers of other normal chromosomes. Eight marker chromosomes are identified: 5p+, t(1pter--->cen::9q12--->9q33::3p21--->3pter), t(1p;?), der(2)t(1;2)(q21;q24), 12p+, der(6)t(6;?)(q21;?), der(4)t(X;4)(q13;q34), del(16)(p13). The cytogenetic and isozyme phenotype descriptors are in accord with those given by J. Fogh.
- Description: SK-LMS-1 is a cell line exhibiting fibroblast morphology that was isolated from the vulva of a 43-year-old, White female patient with leiomyosarcoma
- Application: 3D cell culture
- Biosafety level: 1
Applications
- Application: 3D cell culture
Handling
- Growth medium: EMEM suplemented with 10% FBS.
- Temperature: 37° C
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium, 95%; DMSO, 5%
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
- Str profiling: Amelogenin: X;CSF1PO: 9,10;D13S317: 12;D16S539: 8,11;D5S818: 11,13;D7S820: 8,9;TH01: 6,7;TPOX: 8,9;vWA: 18,20;D3S1358: 15,16;D21S11: 28,30;D18S51: 14,19;Penta_E: 7,13;Penta_D: 12,13;D8S1179: 12;FGA: 22,25;D19S433: 14,15;D2S1338: 17,24
