Cat. #151463
Omega-E Packaging Cell Line
Cat. #: 151463
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Embryo
Model: Packaging
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Hartmut Land, Jay Morgenstern
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Omega-E Packaging Cell Line
- Research fields: Genetics
- Tool sub type: Continuous
- Parental cell: NIH/3T3
- Organism: Mouse
- Tissue: Embryo
- Growth properties: Recombinant retroviral production
- Model: Packaging
- Conditional: Yes
- Description: Helper-free packaging cell line developed by Morgenstern and Land in 1990 for safe, high-titre pBABE vector production. Features split gag-pol/env genes that minimise replication competent virus production, while producing consistent viral titers of 10^6-10^7 cfu/ml. Key component of the proven pBABE retroviral system for custom gene expression experiments. Use with any of the pBABE vectors (puro, neo, hygro and zeo) for complete retroviral gene delivery capabilities.
- Production details: The Omega E and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (Omega E) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer
- Cellosaurus id: CVCL_2151
Target Details
- Target: pBabe vector
Handling
- Format: Frozen
- Growth medium: For recommended growth and recombinant retrovirus production conditions, see Morgenstern & Land. 1990. Nucleic Acids Research. 18:3587-96. PMID: 2194165
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Littlewood et al. 1995. Nucleic Acids Res. 23(10):1686-90. PMID: 7784172.
- Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
