Cat. #161754
MSS-AS22 cell line
Cat. #: 161754
Organism: Human
Tissue: Ascites
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ziv Shulman, Irit Sagi, Roei Mazor
Institute: Weizmann Institute of Science
Primary Citation: Mazor et. al.. Cell, 2022. Mar 31,185(7):1208-1222. PMID: 35305315
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MSS-AS22 cell line
- Alternate name: AS22
- Cancer: Gynaecologic cancer
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Metabolism
- Organism: Human
- Gender: Female
- Tissue: Ascites
- Disease: Cancer
- Morphology: Polymorphic trapezoid cells arrenging in a cobblestone appearance with large cytoplasmic surface area
- Growth properties: Adherent
- Crispr: No
- Products or characteristics of interest: p53 mutant, EpCAM(+)
- Description: The cell line established directly from a tumor compartment (ascites) of chemonaive HGSOC patient. Thus it closely recapitulates the tumor compartment as it emerged in these patients.
- Application: Flow cytometry, Immunofluoresence staining and confocal microscopy
- Production details: Fresh HGSOC ascites sample was retrieved from the operating theatre. Primary culture was established from this specimen as previously described (O Donnell et al. 2014) and used after 2-3 passages. Cell preparation included removing fibroblasts as well as all non-adherent cells from the culture. 100% of the cultured cells were EpCAM positive (324207, Biolegend, 1:200).
- Biosafety level: 1
- Recommended controls: Healthy fallopian tube epiuthelium
Applications
- Application: Flow cytometry, Immunofluoresence staining and confocal microscopy
Handling
- Volume: Frozen
- Passage number: P2-3
- Growth medium: DMEM media supplemented with 10% (v/v) foetal bovine serum, 1% (v/v) MEM-Eagle non essential amino acids, 1% (v/v) 2mM glutamine and 1% (v/v) Pen-Strep solution
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Storage medium: FBS + 10% (v/v) DMSO
- Storage conditions: Liquid Nitrogen
- Initial handling information: Thaw rapidly in preheated 37 °C growth media, centrifuge for 4 minutes in 300G, discard media, re-suspend and plate in preheated 37 °C growth media
- Subculture routine: Slow growing cell line (doubeling time is roughly 96-120 hours). Trypsinize for 3-4 minutes in 37C until cells separate, add growth media (X4 volumes of trypsin). Split 1:2. Resuspend in growth media
- Cultured in antibiotics: Penicillin / streptomycin
- Characterisation tests: In flow cytometry, 100% of the cultured cells were EpCAM positive (324207, Biolegend, 1:200). See tab "EpCAM validation"
References
- Mazor et. al.. Cell, 2022. Mar 31,185(7):1208-1222. PMID: 35305315
