Melan-lnk4a-Arf-b1 cell line

immortal melanocyte cell lines derived from the skin of neonatal Tyrp1b/Tyrp1b Ink4aArf -/- C57BL/6J mice

Contributors

Inventor Institute
Elena Sviderskaya St. George's University London
Cat. #: 162005
Organism: Mouse
Tissue: Skin
Model: Immortalised non-cancerous cell lines
Morphology: Small, oval-fusiform
Primary citation: Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20;94(6):446-54. PMID: 11904317
Product description: The melan-Ink4a-Arf-b1, -b2 and -b3 cell lines are immortal melanocyte cell lines derived from the skin of neonatal Tyrp1b/Tyrp1b Ink4aArf -/- C57BL/6J mice. These melanocytes do not express the melanogenic enzyme tyrosinase-related protein 1 (Tyrp1), or p16Ink4A and Arf. These melanocytes display brown pigmentation in culture which is reduced compared to cell lines that have functional Tyrp1 but are derived from Ink4aArf -/- C57BL/6J mice (such as melan-Ink4a-Arf-1,-2, -3 and -4 which are also available from CancerTools.org. These melanocytes can be used as a model of Tyrp1-null skin melanocytes in studies focussing on skin cancers and melanomas. They are also a useful tool for studies of pigmentation genetics.
Storage conditions: liquid N2
Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
References:

Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20

94(6):446-54. PMID: 11904317

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