MEF ULK2 KO (SIM) Cell Line

MEF ULK2 KO (SIM) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk1

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151776
Product description: The MEF ULK2 KO (SIM) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk1 KO cell lines. Mouse embryonic fibroblast Ulk2 knock-out cell line is derived from an embryo with a mixed genetic background (Blk6/129B Agouti) similar to MEF Ulk1 Ulk2 DKO cell line.
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 +/+ Ulk2 -/- and the MEFs that were isolated and cultured were immortalised SIM using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A54
Disease: Cancer
Cat. #: 151776
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Images

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MEF ULK2 KO (SIM) Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC).

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