MCF7/TAMR-7 Cell line

The MCF7/TAMR-7 Cell line is a breast cancer cell line resistant to tamoxifen. The MCF7/TAMR-7 cell line is a good model cell to study the signalling pathways, which are the major drivers of tamoxifen-resistant growth.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152087
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer;Breast cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Cancer cell line
Growth properties: Adherent
Alternate name: MCF-7/TAMR-7; TAMR-7
Product description: MCF7/TAMR-7 cell line is a stable tamoxifen-resistant subline. The cell line has been established in tissue culture after long term treatment with 1 uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced as an adaptation of an original cell line (MCF7/S0.5), as a model cell system to study the effects of tamoxifen resistant cancer growth. MCF7/TAMR-7 cells are oestrogen receptor positive and progesterone receptor negative. Previous applications of this cell line include treatment with steroidal antiestrogens. MCF7/TAMR-7 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance.
Gender: Female
CRISPR: Yes
Conditional: Yes
Production details: The parental cell line for the MCF7/TAMR-7 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-7 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
Cellosaurus ID: CVCL_1D43
Parental cell line: MCF7 S0.5
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Phenol red-free DMEM:Ham's F-12 containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
Subculture routine: Split 1:30 weekly with Trypsin-EDTA for detachment at 37 °C for 5 minutes. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: MCF7-S0.5 parental line
Mycoplasma free: Yes
References:

Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.

Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577.

Thrane et al. 2015. Oncogene. 34:4199–4210. PMID: 25362855.

Pedersen et al. 2014. Int J Oncol. 45(5):2167-2175. PMID: 25175082.

Lundqvist et al. 2014. Steroids. 85:30-35. PMID: 24747771.

Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470.

Larsen et al. 2012. Int J Oncol. 41(5):1863-1870. PMID: 22961366.

Frankel et al. 2007. Breast Cancer Res Treat. 104(2):165-179. PMID: 17061041.

Berstein et al. 2003. Endocr Relat Cancer. 10(2):267-277. PMID: 12790788.

Images

View Gallery
MCF7/TAMR-7 Cell Line. Mid log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
MCF7/TAMR-7 Cell Line. Late log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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