J558L Cell Line secreting OX40L

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: J558L Cell Line secreting OX40L
• Alternate name: OX4L ligand, CD134, dendritic cells, DCs, Th2, CD525, systemic lupus erythematosus, TXGP1, OX-4L, gp34, TNFSF4, CD4 T cells, cytokine, Tumor necrosis factor receptor superfamily member 4, TNFRSF4
• Cancer type filter: Blood cancer
• Research fields: Immunology
• Tool sub type: Continuous
• Parental cell: J558L - mammalian plasmacytoma cell line
• Organism: Mouse
• Conditional: No
• Description: OX40 receptor also known as CD134, a member of the tumor necrosis factor receptor superfamily of receptors, is not constitutively expressed on resting naive T cells. OX40 is activated 24 to 72 hours after binding to its ligand; OX40L, and is a secondary co-stimulatory immune checkpoint molecule. Expression of OX40 is dependent on full activation of the CD28 absent-T cell.
• Production details: Primers specific to the murine sequence of the OX40L gene were used to generate a cDNA amplicon which was cloned into an expression vector. The vector has resistant markers to ampicillin and histidinol, and also contains promoters and enhancers for murine B-cells and plasma cells. A chimeric IgG1 and OX40 molecule with a 3' splice donor site was generated by subcloning the OX40L amplicon into a human IgG1 expression vector.
• Biosafety level: 1

Target Details

• Target: Murine OX40L

Handling

• Format: Frozen
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042